… Ambient temperature was maintained at 24.5 C for all studies using a Phenomaster climate
chamber. All experimental groups were BW matched upon the start of metabolic assessment …

… (C) Metascape pathway analysis of dysregulated genes in the DRG. (D) Metascape pathway
analysis of dysregulated genes in the SN. FDR-adjusted P < 0.05 (n = 2 biological replicates…

… A 4 h 4 C cold exposure test was performed 8 weeks postsurgically to identify whole-animal …
staining, sections were incubated overnight at 4 C with 1:300 polyclonal rabbit anti-TH (…

… thermal cycling at 25C for 10 min, 48C for 30 min, 95C for 5 min, and holding at 4C. The cDNA
… The relative expression was calculated using comparative C T method (ΔC T ). Data are …

… obesityinduced pain is explored by Gavini et al. using cell-… Increased expression of the ER
stress marker C/EBP … compared with control mice (Figures 1C and 1D). Compared with NC-…

Key points The ventromedial hypothalamus (VMH) and the central melanocortin system both
play vital roles in regulating energy balance by modulating energy intake and utilization. …

… C ± 0.3C; paired t-test, P < 0.01) compared to pre-CR baseline temperatures of 37.4C ± 0.3C
(… weights of 596.2g ± 12.9 g: 37.4C ± 0.2C). Within condition, muscle temperature was not …

… 6 month (n = 6), 12 month (n = 6), vehicle or GW3965 treated 21–24 month-old mice (n =
10–12/group) were evaluated in a quiet room, at a constant temperature of 24 C and acclimated …

… ), and rotated overnight at 4 C. Overnight samples were incubated with 25 μl of protein A/G
magnetic beads (Thermo Fisher, #88803) rotating for 4 h at 4 C. Proteins were eluted from the …

… Cells were plated onto poly-l-lysine treated 12-well plates and maintained in a 37 C and 5%CO2
incubator for 4 days with media changes supplemented with Ara-C (2 μM) to inhibit non-…